Background/ Objective: Universal precautions are a major component of infectious disease control in any health facility. The main objective of this study is to determine the association between the availability of personal protective equipment, the training of health workers, and the existence of policies on universal precautions among health workers in Umuahia North and Umuahia South Local Government Areas (LGAs) of Abia State, Nigeria.

Methods: A descriptive cross-sectional research design among 300 randomly selected health workers from 12 randomly selected health facilities in the two research LGAs. The research instruments were structured questionnaires. Data analysis was done using the Statistical Package for Social Sciences (SPSS) version 25. Frequency tables were used for descriptive statistics, while logistic regression was used for the inferential statistics.

Results: In terms of exposure to training on universal precaution, the study found out that those who had training once did not make much use of universal precaution (AOR 0.92, p = 0.001), but with twice or more training, the health workers made more use of universal precaution (AOR = 1.04, p = 0.050). Policies on universal precautions were available in all the assessed facilities. Participants who had hand gloves available were more likely to use them than those that did not have them available (AOR = 2.05, ρ = 0.03). Those who had antiseptics and running water were more likely to use them than those who did not (AOR = 2.39, ρ = 0.001). Those who have universal precaution policies in their facilities were more likely to apply universal precautions than those who did not (AOR = 2.94, ρ = 0.002).

Conclusion: It is concluded that health workers will apply universal precautions while carrying out their duties if they have personal protective equipment, antiseptics, running water, adequate training, and a sound policy in place.